Recombinant:Article Title: Molecular insights into the effect of 1,6-hexanediol on FUS phase separation.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Recombinant DNA pTHMT FUS 1-526, AddGene cat # 98651 pRP1B FUS LC-RGG1 (1-284) Fawzi Lab pRP1B_FUS_LCRGG1 pTHMT FUS RGG3 (453-507) Fawzi Lab pTHMT_FUS_RGG3 Oligonucleotides and other sequence-based reagents Poly-L-lysine hydrochloride Alamanda Polymers cat # 26124 Poly-L-aspartic acid sodium salt Alamanda Polymers cat # 34345 Polyadenylic acid potassium salt Sigma-Aldrich cat # P9403 Polyuridylic acid potassium salt Sigma-Aldrich cat # P9528 Reagent/Resource Reference or Source Identifier or Catalog Number Torula yeast RNA extract Sigma-Aldrich cat # R6625 Chemicals, Enzymes and other reagents 1,2-hexanediol Sigma-Aldrich cat # 213691 1,6-cyclohexanediol Sigma-Aldrich cat #141712 1,6-hexanediol Sigma-Aldrich cat # 240117 2,5-hexanediol Sigma-Aldrich cat # H11904 1,4-butanediol Sigma-Aldrich cat # 493732 1,5-pentanediol Sigma-Aldrich cat # P7703 Lysozyme Sigma-Aldrich cat # L4919 Software NMRPipe (Delaglio et al, 1995) CCPNmr Analysis (Vranken et al, 2005) NMRFAM-Sparky (Lee et al, 2015) Fiji (ImageJ) (Schindelin et al, 2012) ACPYPE python package (Sousa da Silva and Vranken, 2012) GAFF2 force field (He et al, 2020) GROMACS-2022 MD package (Abraham et al, 2015) AMBER03ws force field (Best et al, 2014) Amber22 package (Case et al, 2022) ParmEd package (Shirts et al, 2017) MDAnalysis-2.5.0 (Gowers et al, 2016) Bruker Topspin 3.2 http://bruker.com/topspin Matlab https://www.mathworks.com/ products/matlab.html Other Fluorescence microscope Zeiss Axiovert 200M NMR spectrometer Bruker Avance III 850MHz CD spectropolarimeter JASCO J-815 .. 1,2-hexanediol (#213691), 1,6-cyclohexanediol (#141712), 1,6- hexanediol (#240117), 2,5-hexanediol (#H11904), 1,4-butanediol (#493732) and 1,5-pentanediol (#P7703) were purchased from Sigma-Aldrich.
Sequencing:Article Title: Molecular insights into the effect of 1,6-hexanediol on FUS phase separation.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Recombinant DNA pTHMT FUS 1-526, AddGene cat # 98651 pRP1B FUS LC-RGG1 (1-284) Fawzi Lab pRP1B_FUS_LCRGG1 pTHMT FUS RGG3 (453-507) Fawzi Lab pTHMT_FUS_RGG3 Oligonucleotides and other sequence-based reagents Poly-L-lysine hydrochloride Alamanda Polymers cat # 26124 Poly-L-aspartic acid sodium salt Alamanda Polymers cat # 34345 Polyadenylic acid potassium salt Sigma-Aldrich cat # P9403 Polyuridylic acid potassium salt Sigma-Aldrich cat # P9528 Reagent/Resource Reference or Source Identifier or Catalog Number Torula yeast RNA extract Sigma-Aldrich cat # R6625 Chemicals, Enzymes and other reagents 1,2-hexanediol Sigma-Aldrich cat # 213691 1,6-cyclohexanediol Sigma-Aldrich cat #141712 1,6-hexanediol Sigma-Aldrich cat # 240117 2,5-hexanediol Sigma-Aldrich cat # H11904 1,4-butanediol Sigma-Aldrich cat # 493732 1,5-pentanediol Sigma-Aldrich cat # P7703 Lysozyme Sigma-Aldrich cat # L4919 Software NMRPipe (Delaglio et al, 1995) CCPNmr Analysis (Vranken et al, 2005) NMRFAM-Sparky (Lee et al, 2015) Fiji (ImageJ) (Schindelin et al, 2012) ACPYPE python package (Sousa da Silva and Vranken, 2012) GAFF2 force field (He et al, 2020) GROMACS-2022 MD package (Abraham et al, 2015) AMBER03ws force field (Best et al, 2014) Amber22 package (Case et al, 2022) ParmEd package (Shirts et al, 2017) MDAnalysis-2.5.0 (Gowers et al, 2016) Bruker Topspin 3.2 http://bruker.com/topspin Matlab https://www.mathworks.com/ products/matlab.html Other Fluorescence microscope Zeiss Axiovert 200M NMR spectrometer Bruker Avance III 850MHz CD spectropolarimeter JASCO J-815 .. 1,2-hexanediol (#213691), 1,6-cyclohexanediol (#141712), 1,6- hexanediol (#240117), 2,5-hexanediol (#H11904), 1,4-butanediol (#493732) and 1,5-pentanediol (#P7703) were purchased from Sigma-Aldrich.
Software:Article Title: Molecular insights into the effect of 1,6-hexanediol on FUS phase separation.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Recombinant DNA pTHMT FUS 1-526, AddGene cat # 98651 pRP1B FUS LC-RGG1 (1-284) Fawzi Lab pRP1B_FUS_LCRGG1 pTHMT FUS RGG3 (453-507) Fawzi Lab pTHMT_FUS_RGG3 Oligonucleotides and other sequence-based reagents Poly-L-lysine hydrochloride Alamanda Polymers cat # 26124 Poly-L-aspartic acid sodium salt Alamanda Polymers cat # 34345 Polyadenylic acid potassium salt Sigma-Aldrich cat # P9403 Polyuridylic acid potassium salt Sigma-Aldrich cat # P9528 Reagent/Resource Reference or Source Identifier or Catalog Number Torula yeast RNA extract Sigma-Aldrich cat # R6625 Chemicals, Enzymes and other reagents 1,2-hexanediol Sigma-Aldrich cat # 213691 1,6-cyclohexanediol Sigma-Aldrich cat #141712 1,6-hexanediol Sigma-Aldrich cat # 240117 2,5-hexanediol Sigma-Aldrich cat # H11904 1,4-butanediol Sigma-Aldrich cat # 493732 1,5-pentanediol Sigma-Aldrich cat # P7703 Lysozyme Sigma-Aldrich cat # L4919 Software NMRPipe (Delaglio et al, 1995) CCPNmr Analysis (Vranken et al, 2005) NMRFAM-Sparky (Lee et al, 2015) Fiji (ImageJ) (Schindelin et al, 2012) ACPYPE python package (Sousa da Silva and Vranken, 2012) GAFF2 force field (He et al, 2020) GROMACS-2022 MD package (Abraham et al, 2015) AMBER03ws force field (Best et al, 2014) Amber22 package (Case et al, 2022) ParmEd package (Shirts et al, 2017) MDAnalysis-2.5.0 (Gowers et al, 2016) Bruker Topspin 3.2 http://bruker.com/topspin Matlab https://www.mathworks.com/ products/matlab.html Other Fluorescence microscope Zeiss Axiovert 200M NMR spectrometer Bruker Avance III 850MHz CD spectropolarimeter JASCO J-815 .. 1,2-hexanediol (#213691), 1,6-cyclohexanediol (#141712), 1,6- hexanediol (#240117), 2,5-hexanediol (#H11904), 1,4-butanediol (#493732) and 1,5-pentanediol (#P7703) were purchased from Sigma-Aldrich.
Fluorescence:Article Title: Molecular insights into the effect of 1,6-hexanediol on FUS phase separation.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Recombinant DNA pTHMT FUS 1-526, AddGene cat # 98651 pRP1B FUS LC-RGG1 (1-284) Fawzi Lab pRP1B_FUS_LCRGG1 pTHMT FUS RGG3 (453-507) Fawzi Lab pTHMT_FUS_RGG3 Oligonucleotides and other sequence-based reagents Poly-L-lysine hydrochloride Alamanda Polymers cat # 26124 Poly-L-aspartic acid sodium salt Alamanda Polymers cat # 34345 Polyadenylic acid potassium salt Sigma-Aldrich cat # P9403 Polyuridylic acid potassium salt Sigma-Aldrich cat # P9528 Reagent/Resource Reference or Source Identifier or Catalog Number Torula yeast RNA extract Sigma-Aldrich cat # R6625 Chemicals, Enzymes and other reagents 1,2-hexanediol Sigma-Aldrich cat # 213691 1,6-cyclohexanediol Sigma-Aldrich cat #141712 1,6-hexanediol Sigma-Aldrich cat # 240117 2,5-hexanediol Sigma-Aldrich cat # H11904 1,4-butanediol Sigma-Aldrich cat # 493732 1,5-pentanediol Sigma-Aldrich cat # P7703 Lysozyme Sigma-Aldrich cat # L4919 Software NMRPipe (Delaglio et al, 1995) CCPNmr Analysis (Vranken et al, 2005) NMRFAM-Sparky (Lee et al, 2015) Fiji (ImageJ) (Schindelin et al, 2012) ACPYPE python package (Sousa da Silva and Vranken, 2012) GAFF2 force field (He et al, 2020) GROMACS-2022 MD package (Abraham et al, 2015) AMBER03ws force field (Best et al, 2014) Amber22 package (Case et al, 2022) ParmEd package (Shirts et al, 2017) MDAnalysis-2.5.0 (Gowers et al, 2016) Bruker Topspin 3.2 http://bruker.com/topspin Matlab https://www.mathworks.com/ products/matlab.html Other Fluorescence microscope Zeiss Axiovert 200M NMR spectrometer Bruker Avance III 850MHz CD spectropolarimeter JASCO J-815 .. 1,2-hexanediol (#213691), 1,6-cyclohexanediol (#141712), 1,6- hexanediol (#240117), 2,5-hexanediol (#H11904), 1,4-butanediol (#493732) and 1,5-pentanediol (#P7703) were purchased from Sigma-Aldrich.
Microscopy:Article Title: Molecular insights into the effect of 1,6-hexanediol on FUS phase separation.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Recombinant DNA pTHMT FUS 1-526, AddGene cat # 98651 pRP1B FUS LC-RGG1 (1-284) Fawzi Lab pRP1B_FUS_LCRGG1 pTHMT FUS RGG3 (453-507) Fawzi Lab pTHMT_FUS_RGG3 Oligonucleotides and other sequence-based reagents Poly-L-lysine hydrochloride Alamanda Polymers cat # 26124 Poly-L-aspartic acid sodium salt Alamanda Polymers cat # 34345 Polyadenylic acid potassium salt Sigma-Aldrich cat # P9403 Polyuridylic acid potassium salt Sigma-Aldrich cat # P9528 Reagent/Resource Reference or Source Identifier or Catalog Number Torula yeast RNA extract Sigma-Aldrich cat # R6625 Chemicals, Enzymes and other reagents 1,2-hexanediol Sigma-Aldrich cat # 213691 1,6-cyclohexanediol Sigma-Aldrich cat #141712 1,6-hexanediol Sigma-Aldrich cat # 240117 2,5-hexanediol Sigma-Aldrich cat # H11904 1,4-butanediol Sigma-Aldrich cat # 493732 1,5-pentanediol Sigma-Aldrich cat # P7703 Lysozyme Sigma-Aldrich cat # L4919 Software NMRPipe (Delaglio et al, 1995) CCPNmr Analysis (Vranken et al, 2005) NMRFAM-Sparky (Lee et al, 2015) Fiji (ImageJ) (Schindelin et al, 2012) ACPYPE python package (Sousa da Silva and Vranken, 2012) GAFF2 force field (He et al, 2020) GROMACS-2022 MD package (Abraham et al, 2015) AMBER03ws force field (Best et al, 2014) Amber22 package (Case et al, 2022) ParmEd package (Shirts et al, 2017) MDAnalysis-2.5.0 (Gowers et al, 2016) Bruker Topspin 3.2 http://bruker.com/topspin Matlab https://www.mathworks.com/ products/matlab.html Other Fluorescence microscope Zeiss Axiovert 200M NMR spectrometer Bruker Avance III 850MHz CD spectropolarimeter JASCO J-815 .. 1,2-hexanediol (#213691), 1,6-cyclohexanediol (#141712), 1,6- hexanediol (#240117), 2,5-hexanediol (#H11904), 1,4-butanediol (#493732) and 1,5-pentanediol (#P7703) were purchased from Sigma-Aldrich.
Nuclear Magnetic Resonance:Article Title: Molecular insights into the effect of 1,6-hexanediol on FUS phase separation.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Recombinant DNA pTHMT FUS 1-526, AddGene cat # 98651 pRP1B FUS LC-RGG1 (1-284) Fawzi Lab pRP1B_FUS_LCRGG1 pTHMT FUS RGG3 (453-507) Fawzi Lab pTHMT_FUS_RGG3 Oligonucleotides and other sequence-based reagents Poly-L-lysine hydrochloride Alamanda Polymers cat # 26124 Poly-L-aspartic acid sodium salt Alamanda Polymers cat # 34345 Polyadenylic acid potassium salt Sigma-Aldrich cat # P9403 Polyuridylic acid potassium salt Sigma-Aldrich cat # P9528 Reagent/Resource Reference or Source Identifier or Catalog Number Torula yeast RNA extract Sigma-Aldrich cat # R6625 Chemicals, Enzymes and other reagents 1,2-hexanediol Sigma-Aldrich cat # 213691 1,6-cyclohexanediol Sigma-Aldrich cat #141712 1,6-hexanediol Sigma-Aldrich cat # 240117 2,5-hexanediol Sigma-Aldrich cat # H11904 1,4-butanediol Sigma-Aldrich cat # 493732 1,5-pentanediol Sigma-Aldrich cat # P7703 Lysozyme Sigma-Aldrich cat # L4919 Software NMRPipe (Delaglio et al, 1995) CCPNmr Analysis (Vranken et al, 2005) NMRFAM-Sparky (Lee et al, 2015) Fiji (ImageJ) (Schindelin et al, 2012) ACPYPE python package (Sousa da Silva and Vranken, 2012) GAFF2 force field (He et al, 2020) GROMACS-2022 MD package (Abraham et al, 2015) AMBER03ws force field (Best et al, 2014) Amber22 package (Case et al, 2022) ParmEd package (Shirts et al, 2017) MDAnalysis-2.5.0 (Gowers et al, 2016) Bruker Topspin 3.2 http://bruker.com/topspin Matlab https://www.mathworks.com/ products/matlab.html Other Fluorescence microscope Zeiss Axiovert 200M NMR spectrometer Bruker Avance III 850MHz CD spectropolarimeter JASCO J-815 .. 1,2-hexanediol (#213691), 1,6-cyclohexanediol (#141712), 1,6- hexanediol (#240117), 2,5-hexanediol (#H11904), 1,4-butanediol (#493732) and 1,5-pentanediol (#P7703) were purchased from Sigma-Aldrich.
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